See
Genome Information for Mus musculus
TGF-beta-responding SCC cells, a tumor-initiating cell population, show a unique gene expression signature, including antioxidant gene expression and IL-33 upregulation. IL-33-responding macrophages in SCC, which also express Fc epsilon receptor I alpha, show a gene expression signature that resembles alternatively-activated macrophages
Overall design: To analyze a unique gene expression in TGF-beta-responding SCC cells, we purified TGF-beta signaling reporter (mScarlet) positive and negative tumor basal cells (Rosa-YFP+ CD49f+ CD29+) by FACS. We used RNA-seq analysis to compare the transcriptomes of these FACS-isolated SCC cells and combined the previous RNA-seq data (GSE64867) to obtain higher confidence (p < 0.01). For macrophage analysis, we purified Fc epsilon receptor I alpha positive and negative macrophages (CD45+ F4/80+) from SCC tumor tissues. We also used RNA-seq analysis to compare their transcriptomes.
| Accession | PRJNA637244; GEO: GSE151783 |
| Data Type | Transcriptome or Gene expression |
| Scope | Multiisolate |
| Organism | Mus musculus[Taxonomy ID: 10090] Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus; Mus musculus |
| Publications | Taniguchi S et al., "Tumor-initiating cells establish an IL-33-TGF-β niche signaling loop to promote cancer progression.", Science, 2020 Jul 17;369(6501) |
| Submission | Registration date: 4-Jun-2020
Oshimori Lab, Cell, Developmental & Cancer Biology, Oregon Health & Science University |
| Relevance | Model Organism |
Project Data:
| Resource Name | Number
of Links |
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| Sequence data |
| SRA Experiments | 12 |
| Publications |
| PubMed | 1 |
| Other datasets |
| BioSample | 12 |
| GEO DataSets | 1 |