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Genome Information for Mus musculus
Purpose: To study the function of Runx2 on mouse tooth root development. The goals of this study are to compare NGS-derived molar transcriptome profiling (RNA-seq) bwtween Runx2fl/fl and Gli1-CreERT2;Runx2fl/fl mice to identify the potential downstream target of Runx2. Methods: molar mRNA profiles from PN7.5 Runx2fl/fl and Gli1-CreERT2;Runx2fl/fl mice (induction at PN3.5) were generated by deep sequencing, using Novaseq. Results: Using an optimized data analysis workflow, we mapped about 68 million sequence reads per sample to the mouse genome ( mm10) and identified 80,214 transcripts with Partek E/M workflow. 427 differentially regulated genes were identified (>1.8-fold, p<0.05), of which 219 were upregulated and 208 were downregulated.
Overall design: Methods: molar mRNA profiles from PN7.5 Runx2fl/fl and Gli1-CreERT2;Runx2fl/fl mice (induction at PN3.5) were generated by deep sequencing, using 2x50 sequencing with Novaseq, each group had 4 samples.
| Accession | PRJNA636368; GEO: GSE151562 |
| Data Type | Transcriptome or Gene expression |
| Scope | Multiisolate |
| Organism | Mus musculus[Taxonomy ID: 10090] Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus; Mus musculus |
| Submission | Registration date: 1-Jun-2020
University of Southern California |
| Relevance | Model Organism |
Project Data:
| Resource Name | Number
of Links |
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| Sequence data |
| SRA Experiments | 8 |
| Other datasets |
| BioSample | 8 |
| GEO DataSets | 1 |