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Genome Information for Homo sapiens
We used two siRNAs to knock down GNL3 in human neural progenitor cells which were derived from normal human induced pluripotent stem cells (ATCC, ACS-1011). GNL3 knockdown experiments were done in three biological replicates. Total RNA was extracted from GNL3 knockdown and control groups for RNA sequencing (Illumina Hiseq2000, paired-end 100 bp). Genes that affected by both siRNAs were considered differentially expressed genes between GNL3 knockdown and control groups (adjusted P value < 0.05). Using Gene Ontology and KEGG pathway analysis, we found that those differentially exrepssed genes were mainly related to immune response, response to cytokine, cell cycle, and p53 signaling pathway.
Overall design: GNL3 was knocked down in human neural progenitor cells by using two siRNAs. RNA-seq was then used to identify genes affected by GNL3 knockdown.
| Accession | PRJNA634131; GEO: GSE150945 |
| Data Type | Transcriptome or Gene expression |
| Scope | Multiisolate |
| Organism | Homo sapiens[Taxonomy ID: 9606] Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo; Homo sapiens |
| Publications | Meng Q et al., "Integrative analyses prioritize GNL3 as a risk gene for bipolar disorder.", Mol Psychiatry, 2020 Nov;25(11):2672-2684 |
| Submission | Registration date: 20-May-2020
Affiliated Hospital of Guilin Medical University, Guilin Medical University |
| Relevance | Medical |
Project Data:
| Resource Name | Number
of Links |
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| Sequence data |
| SRA Experiments | 9 |
| Publications |
| PubMed | 1 |
| Other datasets |
| BioSample | 9 |
| GEO DataSets | 1 |