BioProject

Next-generation massively parallel sequencing technologies and de novo transcriptome assembly was used to gain insight into the wide range of transcriptome of Hevea brasiliensis. More...

Next-generation massively parallel sequencing technologies and de novo transcriptome assembly was used to gain insight into the wide range of transcriptome of Hevea brasiliensis. The output of sequenced data showed that more than 12 million sequence reads with average length of 90nt were generated. Totally 48,768 unigenes (mean size = 488 bp) were assembled through transcriptome de novo assembly, which represent more than 3-fold of all the sequences of Hevea brasiliensis deposited in the GenBank (as of Oct 2010). Assembled sequences were annotated with gene descriptions, gene ontology and clusters of orthologous group terms. 37,373 unigenes were successfully annotated and more than 10% of unigenes were aligned to known proteins of Euphorbiaceae. The unigenes contain nearly complete collection of known rubber-synthesis-related genes. Our data provides the most comprehensive sequence resource available for rubber tree and demonstrates the availability of Illumina sequencing and de novo transcriptome assembly in a species lacking genome information. Less...