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Genome Information for Homo sapiens
We report that differential gene expression analysis of epithelioid sarcoma samples removed from EPZ-011989-treated and untreated mice revealed that, among the 5521 genes with a significant differential expression (according to FDR<0.05), 62% were up-regulated and 38% down-regulated respectively, thus indicating a generally increased transcriptional activity in treated tumors, consistent with EZH2 inhibition. Interestingly, among the top 15 up-regulated genes in the EPZ-011989-treated samples, 8 are involved in cell survival pathways, including autophagy. The induction of autophagy as a fail-safe mechanism against EZH2 inhibition was further validated in the PDX-derived ES-1 cell line by evaluating the effect of autophagy inhibition on drug-induced activity.
Overall design: PDX mRNA profiles of untreated and EPZ-011989 treated mice were generated by deep sequencing, in duplicate using Illumina NextSeq 500 Sequencing System
| Accession | PRJNA522084; GEO: GSE126495 |
| Data Type | Transcriptome or Gene expression |
| Scope | Multiisolate |
| Organism | Homo sapiens[Taxonomy ID: 9606] Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo; Homo sapiens |
| Publications | Stacchiotti S et al., "Comparative Assessment of Antitumor Effects and Autophagy Induction as a Resistance Mechanism by Cytotoxics and EZH2 Inhibition in INI1-Negative Epithelioid Sarcoma Patient-Derived Xenograft.", Cancers (Basel), 2019 Jul 19;11(7) |
| Submission | Registration date: 13-Feb-2019
AmadeoLab, DRAST, Fondazione IRCCS - Istituto Nazionale dei Tumori |
| Relevance | Medical |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 4 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 4 |
| GEO DataSets | 1 |