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Genome Information for Saccharomyces cerevisiae
In this study, we performed RNAseq of PolyA enriched RNA from budding yeast cells that were either wild-type or having the mcd1-1 mutation. By obtaining over 200 million reads of sequences from PolyA RNA, we mapped greater than 30 million reads per sample to the yeast genome (S288C R64-2-1_20150113)and generated transcriptomic profiles of wild-type and mcd1-1 mutant cells. Comparison of the wild type and mutant transcriptomes revealed that 11.44% genes were upregulated, 4.99% were downregulated and 83.57% were unaffected in the mutant with a fold change ≥2.0 and Padj value <0.05. When the alterations in gene expression were analyzed with respect to chromosomal position, preferential derepression of subtelomeric (located within 20 kilo bases from the chromosome end) genes was observed in the mutant.
Overall design: PolyA mRNA profiles of wild type (WT) and mcd1-1 budding yeast cells were generated by deep sequencing, in quadruplicate, using Illumina HiSeq X.
| Accession | PRJNA521702; GEO: GSE126364 |
| Data Type | Transcriptome or Gene expression |
| Scope | Multiisolate |
| Organism | Saccharomyces cerevisiae[Taxonomy ID: 4932] Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces; Saccharomyces cerevisiae |
| Publications | Kothiwal D et al., "A SIR-independent role for cohesin in subtelomeric silencing and organization.", Proc Natl Acad Sci U S A, 2019 Mar 19;116(12):5659-5664 |
| Submission | Registration date: 11-Feb-2019
FE13, Biochemistry, Indian Instritute of Science |
| Relevance | Model Organism |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 8 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 8 |
| GEO DataSets | 1 |