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Genome Information for Homo sapiens
We used CRISPR/Cas9 system to generate WT Control and RUNX1-KO MDS-L cells isolated as single clones from the parental MDS-L cell line. We have observed that RUNX1-KO MDS-L cells are resistant to Lenalidomide-induced cell death compared to WT Control MDS-L cells
We have treated WT and RUNX1-KO MDS-L cells with 1µM Lenalidomide for 24h or 72h and performed expression analysis
Overall design: The experiment consists in eight groups in total. Each group was analyzed with three technical replicates. The groups (per timepoint) are as follows: MDS-L WT DMSO; MDS-L WT LEN; MDS-L KO DMSO; MDS-L KO LEN. We then compared the transcriptional response to LEN within each genotype (MDS-L WT or MDS-L RUNX1-KO).Genes with padj<0.05 we identified as differentially expressed.
| Accession | PRJNA521440; GEO: GSE126265 |
| Data Type | Transcriptome or Gene expression |
| Scope | Multiisolate |
| Organism | Homo sapiens[Taxonomy ID: 9606] Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo; Homo sapiens |
| Publications | Martinez-Høyer S et al., "Loss of lenalidomide-induced megakaryocytic differentiation leads to therapy resistance in del(5q) myelodysplastic syndrome.", Nat Cell Biol, 2020 May;22(5):526-533 |
| Submission | Registration date: 7-Feb-2019
Genome Sciences Centre, BC Cancer Agency |
| Relevance | Medical |
Project Data:
| Resource Name | Number
of Links |
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| Publications |
| PubMed | 1 |
| Other datasets |
| GEO DataSets | 1 |
GEO Data Details| Parameter | Value |
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| Data volume, Spots | 1185480 |
| Data volume, Processed Mbytes | 26 |
| Data volume, Supplementary Mbytes | 50 |