BioProject

Streptococcus pneumoniae is the most frequent cause of community-acquired pneumonia. More...

Streptococcus pneumoniae is the most frequent cause of community-acquired pneumonia. Endogenous antimicrobial proteins such as PGLYRP4 might participate to the course of the disease. PGLYRP4 is expressed in different cell types but is downregulated in alveolar epithelial after stimulation with Streptococcus pneumoniae. Interestingly, mice lacking PGLYRP4 displayed an enhanced bacterial clearance in the lungs and less mice develop a bacteremia. We investigated the gene expression in alveolar macrphages and found most top 50 up- and down-regulated genes to be not annotated. Genes which could be annotated seem unlikely to play a major role in the phenotype of PGLYRP4-KO mice. We implemented microarray analysis of infected primary alveolar macrophage cells to identify possible regulated genes by PGLYPR4-deficency, which contribute to the seen phenotype. Overall design: We isolated primary alveolar macrophages cells from untreated and S. pneumoniae-infected 10-16 week old BALB/c wt and PGLYRP4-deficient mice. We used three mice of each strain and performed three indipendant experiments. Less...