BioProject

The role played by transcription factors in the regulation of strawberry fruit ripening process is scant. More...

The role played by transcription factors in the regulation of strawberry fruit ripening process is scant. We have identified and functionally characterized FaPRE1, a non-DNA-binding bHLH transcription factor. FaPRE1 is a ripening-related transcription factor that regulates genes involved in cell architecture in strawberry fruit receptacles. Overall design: A custom-made oligo microarray (60-mer length) platform (FraGenomics 35k) containing a total of 35234 singletons corresponding to those sequences published in the strawberry genome project (http://www.strawberry.org). For each sequence, 4 different oligos were printed per block and four blocks were printed per data set. We have analyzed three control samples fruits agroinjected with the empty vector, a three samples fruits agroinjected with the RNAi construct. Please note that experiments were carried out technically as dual channel (eg, Cy3 and Cy5-labeled samples hybridized to the same array) but processed the results as though they are single channel (Cy3 and Cy5 signals are calculated; Cy3/Cy5 ratios are not calculated). Therefore 3 raw data files for total 6 samples are linked as Series supplementary files and the associated raw data file is indicated in the corresponding sample description field. Less...