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Accession: PRJDA51849 ID: 51849

Escherichia coli K-12 strain:BW25113

Escherichia coli K-12 Transcriptome project

See Genome Information for Escherichia coli
First, Escherichia coli cells were grown to late exponential phase (O.D.600 = 0.76) in modified M63 medium. Then, low molecular weight RNAs (<200 nt) were isolated from the cultured cells using mirVana miRNA Isolation Kit (Ambion). cDNA library was built from the RNA sample ligated with both 5' and 3' end specific RNA adapters for determining direction of each transcript. The cDNA library was deep sequenced by Illumina/Solexa 1G Genome Analyzer system.
AccessionPRJDA51849
Data TypeTranscriptome or Gene expression
ScopeMonoisolate
OrganismEscherichia coli K-12[Taxonomy ID: 83333]
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia; Escherichia coli; Escherichia coli K-12
PublicationsShinhara A et al., "Deep sequencing reveals as-yet-undiscovered small RNAs in Escherichia coli.", BMC Genomics, 2011 Aug 24;12:428
SubmissionRegistration date: 1-Sep-2010
Keio University
Locus Tag PrefixEK3
Project Data:
Resource NameNumber
of Links
Sequence data
SRA Experiments1
Publications
PubMed1
PMC1
Other datasets
BioSample1
SRA Data Details
ParameterValue
Data volume, Mbases436
Data volume, Mbytes1267

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