The enteric nervous system (ENS) predominantly originates from vagal neural crest cells (VNC) that emerge from the caudal hindbrain, invade the foregut and populate the gastrointestinal tract. However, the gene regulatory network (GRN) orchestrating the early specification of VNC remains unknown. Using an EdnrB enhancer, we generated a comprehensive temporal map of the chromatin and transcriptional landscape of VNC in the avian model, revealing three VNC cell clusters (neural, neurogenic and mesenchymal), each predetermined epigenetically prior to neural tube delamination. We identify and functionally validate regulatory cores (Sox10/Tfap2B/SoxB/Hbox) mediating each programme and elucidate their combinatorial activities with other spatiotemporally-specific transcription factors (bHLH/NR).
More...The enteric nervous system (ENS) predominantly originates from vagal neural crest cells (VNC) that emerge from the caudal hindbrain, invade the foregut and populate the gastrointestinal tract. However, the gene regulatory network (GRN) orchestrating the early specification of VNC remains unknown. Using an EdnrB enhancer, we generated a comprehensive temporal map of the chromatin and transcriptional landscape of VNC in the avian model, revealing three VNC cell clusters (neural, neurogenic and mesenchymal), each predetermined epigenetically prior to neural tube delamination. We identify and functionally validate regulatory cores (Sox10/Tfap2B/SoxB/Hbox) mediating each programme and elucidate their combinatorial activities with other spatiotemporally-specific transcription factors (bHLH/NR). Our global deconstruction of the VNC-GRN in vivo sheds light on critical early regulatory mechanisms that may influence the divergent neural phenotypes in enteric neuropathies.
Overall design: This SuperSeries is composed of the SubSeries listed below.
Refer to individual Series.
Less...| Accession | PRJNA517324; GEO: GSE125711 |
| Type | Umbrella project |
| Publications | - Lukoseviciute M et al., "Tissue-Specific In Vivo Biotin Chromatin Immunoprecipitation with Sequencing in Zebrafish and Chicken.", STAR Protoc, 2020 Sep 18;1(2):100066
- Ling ITC et al., "Early chromatin shaping predetermines multipotent vagal neural crest into neural, neuronal and mesenchymal lineages.", Nat Cell Biol, 2019 Dec;21(12):1504-1517
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| Submission | Registration date: 27-Jan-2019
Sauka-Spengler lab, University of Oxford, MRC Weatherall Institute of Molecular Medicine |
| Relevance | Superseries |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 67 |
| Publications |
| PubMed | 2 |
| PMC | 2 |
| Other datasets |
| BioSample | 67 |
| GEO DataSets | 6 |
Early chromatin shaping predetermines multipotent vagal neural crest into neural, neuronal and mesenchymal lineages encompasses the following 5 sub-projects:
| Project Type | Number of Projects |
| Epigenomics | 1 |
BioProject
accession | Organism | Title |
|---|
| PRJNA517320 | Gallus gallus | Early chromatin shaping predetermines multipotent vagal neural crest into neural, neuronal and mesenchymal lineages (Sauka-Spengler lab, University...) |
|
| Other | 1 |
BioProject
accession | Organism | Title |
|---|
| PRJNA544618 | Gallus gallus | Early chromatin shaping predetermines multipotent vagal neural crest into neural, neuronal and mesenchymal lineages [RNA-seq and ChIP-seq] (Sauka-Spengler lab, University...) |
|
| Transcriptome or Gene expression | 3 |
BioProject
accession | Organism | Title |
|---|
| PRJNA517325 | Gallus gallus | Early chromatin shaping predetermines multipotent vagal neural crest into neural, neuronal and mesenchymal lineages [CRISPR RNA-seq] (Sauka-Spengler lab, University...) | | PRJNA517322 | Gallus gallus | Early shaping of the chromatin landscape specifies vagal neural crest into distinct lineages [RNA-seq] (Sauka-Spengler lab, University...) | | PRJNA565680 | Gallus gallus | Early chromatin shaping predetermines multipotent vagal neural crest into neural, neuronal and mesenchymal lineages [scRNA-seq] (Sauka-Spengler lab, University...) |
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