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Genome Information for Homo sapiens
This study combined ChIP-seq using two different GR antibodies and stable GR knockdown in Beas-2B airway epithelial cells to determine whether glucocorticoid-mediated repression of inflammatory enhancers requires local GR occupancy
Overall design: Duplicate samples of Beas-2B cells transduced with lentiviral-shRNA against GR (shGR) or a control construct (shCtrl) were treated with vehicle (ethanol), TNF (20 ng/ml), dexamethasone (100 nM) or TNF+dex for one hour prior to chromatin immunoprecipitation with one of two GR antibodies and sequencing. Duplicate Input DNA samples, representing both shCtrl and shGR groups, were included as controls.
| Accession | PRJNA516938; GEO: GSE125623 |
| Data Type | Epigenomics |
| Scope | Multiisolate |
| Organism | Homo sapiens[Taxonomy ID: 9606] Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo; Homo sapiens |
| Publications | Sasse SK et al., "Nascent transcript analysis of glucocorticoid crosstalk with TNF defines primary and cooperative inflammatory repression.", Genome Res, 2019 Nov;29(11):1753-1765 |
| Submission | Registration date: 24-Jan-2019
Anthony Gerber, Medicine, National Jewish Health |
| Relevance | Medical |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 36 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 36 |
| GEO DataSets | 1 |