BioProject

Eukaryotic transcription factors (TFs) form complexes with various partner proteins to recognize their genomic target sites. More...

Eukaryotic transcription factors (TFs) form complexes with various partner proteins to recognize their genomic target sites. Yet, how the DNA sequence determines which TF complex forms at any given site is poorly understood. Here, we demonstrate that high-throughput in vitro DNA binding assays coupled with unbiased computational analysis provide unprecedented insight into how different DNA sequences select distinct compositions and configurations of homeodomain TF complexes. Using inferred knowledge about minor groove width readout, we design targeted protein mutations that destabilize homeodomain binding both in vitro and in vivo in a complex-specific manner. By performing parallel systematic evolution of ligands by exponential enrichment sequencing (SELEX-seq), chromatin immunoprecipitation sequencing (ChIP-seq), RNA sequencing (RNA-seq), and Hi-C assays, we not only classify the majority of in vivo binding events in terms of complex composition but also infer complex-specific functions by perturbing the gene regulatory network controlled by a single complex. Overall design: SELEX-seq was performed on different combinations of the three homeodomain transcription factors Deformed, Homothorax and Extradenticle for both wild-type and shape-readout-impaired mutant protein. In addition, ATAC-seq and ChIP-seq was performed in D.melanogaster 3rd instar imaginal wing discs against both wild-type and shape-readout mutant Extradenticle, as well as Homothorax and Antennapedia TFs. Intrinsic DNA Shape (Zhou, PNAS, 2015) was correlated with TF binding affinity to identify position-specific shape readout (minor groove readout). Amino acid mutations abolishing the identified shape readout were subsequently used in vitro and in vivo to probe the degree to which binding mechanism are recapitulated in vivo. Further Hi-C and RNA-seq data was used to dissect the regulatory network of HD TF complexes and identify complex-specific gene function. Less...