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Organizing biological data
Methods: Women ≥20 years were recruited during pregnancy and milk samples were collected at 3 months post-partum (ClinicalTrials.gov Identifier: NCT01405547). Demographic data, weight (self-reported), height and a 3-hour oral glucose tolerance test were conducted in late pregnancy (30 weeks’ gestation [95% CI 25-33 weeks]). Metagenomic DNA extraction and 16S ribosomal RNA (rRNA) gene sequencing of the V4 hypervariable region using an Illumina MiSeq platform was carried out on 113 milk samples. Less...
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