Peripheral blood (about 10 ml) of healthy female Nili Ravi buffaloes (n=2 per group; total three groups i.e. Tuberculosis-positive, Metritis-positive, and Healthy) was immediately used for total RNA extraction, using the mirVana RNA isolation kit (Ambion, Life Technologies, USA). The total RNA samples were checked for quality and RIN and the qualifying samples were reverse transcribed to cDNA using High-Capacity cDNA Reverse transcription kit (Applied Biosystems, USA) and then outsourced to AgriGenome Labs Private Limited (Kochi, Kerala, India) for next-generation sequencing. The transcriptome samples were sequenced using Illumina Hiseq 2500 platform.
The uqality of the reads are adumbrated below:
Sample Name,# of raw reads (paired-end),# of bases (Gb),GC %,%of data >=Q30,Raw read length (bp)
TB_1,"27,074,735",5.4,51.11,93.8,100x2
TB_2,"27,233,730",5.4,51.44,94,100x2
Metritis_1,"25,307,169",5,49.41,92.8,100x2
Metritis_2,"26,758,839",5.3,52.56,95,100x2
Healthy_1,"26,647,077",5.3,52.29,95.5,100x2
Healthy_2,"27,316,174",5.4,52.66,95.4,100x2 Less...