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Genome Information for Homo sapiens
We used mammosphere formation assay and label-retention assay as functional cellular approaches to enrich for cells with different degree of cancer stem cell properties in the breast cancer cell line MDA-MB-231 and performed single-cell RNA sequencing
Overall design: Single cells from three different populations: 30 cells from G1 cell cycle phase cultured in adherent conditions, 46 cells with low proliferation cultured in non-adherent conditions (mammosphere assasy), 45 cells with high proliferation cultured in non-adherent conditions (mammosphere assay)
| Accession | PRJNA514740; GEO: GSE124989 |
| Data Type | Transcriptome or Gene expression |
| Scope | Multiisolate |
| Organism | Homo sapiens[Taxonomy ID: 9606] Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo; Homo sapiens |
| Publications | - Frontiers Production Office. "Erratum: Identification of Breast Cancer Stem Cell Related Genes Using Functional Cellular Assays Combined With Single-Cell RNA Sequencing in MDA-MB-231 Cells.", Front Genet, 2020;11:84
- Jonasson E et al., "Identification of Breast Cancer Stem Cell Related Genes Using Functional Cellular Assays Combined With Single-Cell RNA Sequencing in MDA-MB-231 Cells.", Front Genet, 2019;10:500
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| Submission | Registration date: 11-Jan-2019
Sahlgrenska Cancer Center, University of Gothenburg |
| Relevance | Medical |
Project Data:
| Resource Name | Number
of Links |
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| Sequence data |
| SRA Experiments | 121 |
| Publications |
| PubMed | 2 |
| PMC | 2 |
| Other datasets |
| BioSample | 121 |
| GEO DataSets | 1 |