We identified two phenotypically and functionally distinct populations in AML-iPSC lines upon hematopoietic differentiation. A cell fraction with a hematopoietic stem cell (HSC) immunophenotype exhibiting adherent growth (iLSCs=samples A) and a fraction of more differentiated cells growing in suspension (iBlasts=samples S).
We used RNA-seq and ATAC-seq analyses to identify molecular differences between the two populations.
Overall design: Comparison of gene expression (RNA-seq) and chromatin accessibility (ATAC-seq) in genetically clonal iLSCs vs iBlasts cells
| Accession | PRJNA514713; GEO: GSE124730 |
| Type | Umbrella project |
| Submission | Registration date: 7-Jan-2019
Icahn School of Medicine at Mount Sinai |
| Relevance | Superseries |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 12 |
| Other datasets |
| BioSample | 12 |
| GEO DataSets | 3 |
Gene expression and chromatin accessibility of iLSCs vs iBlasts cells encompasses the following 2 sub-projects:
| Project Type | Number of Projects |
| Epigenomics | 1 |
BioProject
accession | Organism | Title |
|---|
| PRJNA514716 | Homo sapiens | Gene expression and chromatin accessibility of iLSCs vs iBlasts cells (Icahn School of Medicine...) |
|
| Transcriptome or Gene expression | 1 |
BioProject
accession | Organism | Title |
|---|
| PRJNA514717 | Homo sapiens | Gene expression and chromatin accessibility of iLSCs vs iBlasts cells (Icahn School of Medicine...) |
|