To understand tissue resident features of memory CD4+ and CD8+ T lymphocytes of the bone marrow and/or spleen according to expressing or not the tissue retention marker CD69, we performed whole transcriptome profiling of ex vivo antigen-specific CD69+ and CD69- memory CD4+ T cells isolated from bone marrow and spleen, and ex vivo CD69+ and CD69- memory CD8+ T cells isolated from bone marrow.
Overall design: For CD4 analysis, 8-week-old C57BL/6 mice were primed at day 0 with LCMV.GP61-80-NP-MSA + poly(I:C) and immunized again at day 14 with LCMV.GP61-80 + poly(I:C). Sixty days after the last immunization mice were sacrificed and LCMV.GP66–77-specific CD69+ and CD69- memory CD4+ T cells were isolated from BM and spleen by FACS. The whole transcriptome profiling of purified cells was performed by RNA-seq.
More...To understand tissue resident features of memory CD4+ and CD8+ T lymphocytes of the bone marrow and/or spleen according to expressing or not the tissue retention marker CD69, we performed whole transcriptome profiling of ex vivo antigen-specific CD69+ and CD69- memory CD4+ T cells isolated from bone marrow and spleen, and ex vivo CD69+ and CD69- memory CD8+ T cells isolated from bone marrow.
Overall design: For CD4 analysis, 8-week-old C57BL/6 mice were primed at day 0 with LCMV.GP61-80-NP-MSA + poly(I:C) and immunized again at day 14 with LCMV.GP61-80 + poly(I:C). Sixty days after the last immunization mice were sacrificed and LCMV.GP66–77-specific CD69+ and CD69- memory CD4+ T cells were isolated from BM and spleen by FACS. The whole transcriptome profiling of purified cells was performed by RNA-seq. For CD8 analysis, single cell suspensions were prepared from bone marrow of 8-week old untreated C57BL/6 male mice. CD8+ T cells were pre-enriched with anti-CD8a microbeads and the CD69+ and CD69- subpopulations of memory CD8+ T cells were then FACS purified. The whole transcriptome profiling of sorted cells was analyzed by microarray analysis.
Less...| Accession | PRJNA513497; GEO: GSE124796 |
| Type | Umbrella project |
| Publications | Siracusa F et al., "CD69+ memory T lymphocytes of the bone marrow and spleen express the signature transcripts of tissue-resident memory T lymphocytes.", Eur J Immunol, 2019 Jun;49(6):966-968 |
| Submission | Registration date: 8-Jan-2019
Deutsches Rheuma-Forschungszentrum |
| Relevance | Superseries |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 12 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 12 |
| GEO DataSets | 3 |
GEO Data Details| Parameter | Value |
|---|
| Data volume, Spots | 270606 |
| Data volume, Processed Mbytes | 7 |
| Data volume, Supplementary Mbytes | 23 |
| Parameter | Value |
|---|
| Data volume, Gbases | 17 |
| Data volume, Mbytes | 6856 |
CD69+ memory T lymphocytes of the bone marrow and spleen express the signature transcripts of tissue-resident memory T lymphocytes encompasses the following 2 sub-projects:
| Project Type | Number of Projects |
| Transcriptome or Gene expression | 2 |
BioProject
accession | Organism | Title |
|---|
| PRJNA513499 | Mus musculus | Whole-transcriptome profiling of murine steady state CD69+ and CD69- memory CD8+ T cells from the bone marrow [expression microarray] (Deutsches Rheuma-Forschungszentrum) | | PRJNA513500 | Mus musculus | Whole-transcriptome profiling of LCMV.GP66-77 specific CD4 T cells isolated from bone marrow (BM) and spleen, 60 days after last immunization [RNA-seq] (Deutsches Rheuma-Forschungszentrum) |
|