BioProject

Endomyocardial biopsy (EMB) is the gold standard for diagnosis of several heart diseases, yet its use is limited by relatively low diagnostic yield and significant complication risks. More...

Endomyocardial biopsy (EMB) is the gold standard for diagnosis of several heart diseases, yet its use is limited by relatively low diagnostic yield and significant complication risks. To address this, we have developed an endovascular micro-biopsy (Micro-EMB) device with an outer diameter of 0.4 mm in order to minimize procedural risk, sampling trauma, and increase sampling specificity. We have performed RNA-seq on the resulting submilligram tissue as well as controls. The gene expression signatures were compared between Micro-EMBs and conventional EMB controls, as well as negative controls such as blood and skeletal muscle. Overall design: 32 micro-biopsy samples from 5 different individual pigs, of which 20 were taken on the naive heart (baseline) and 12 were taken one week after (follow-up). 10 heart control samples, of which 6 baseline and 4 follow-up, from 2 different animals. 6 blood samples at baseline, from 2 different animals. 6 skeletal muscle samples at baseline, from 2 different animals. Less...