The RNA-directed DNA methylation (RdDM) pathway in plants controls gene expression via cytosine DNA methylation. The ability to manipulate RdDM would shed light on the mechanisms and applications of DNA methylation to control gene expression. Here, we identified diverse RdDM proteins that are capable of targeting methylation and silencing in Arabidopsis when tethered to an artificial zinc finger (ZF-RdDM). We studied their order of action within the RdDM pathway by testing their ability to target methylation in different mutants. We also evaluated ectopic siRNA biogenesis, RNA Polymerase V (Pol V) recruitment, targeted DNA methylation, and gene expression changes at thousands of ZF-RdDM targets. We found that co-targeting both arms of the RdDM pathway, siRNA biogenesis and Pol V recruitment, dramatically enhanced targeted methylation.
More...The RNA-directed DNA methylation (RdDM) pathway in plants controls gene expression via cytosine DNA methylation. The ability to manipulate RdDM would shed light on the mechanisms and applications of DNA methylation to control gene expression. Here, we identified diverse RdDM proteins that are capable of targeting methylation and silencing in Arabidopsis when tethered to an artificial zinc finger (ZF-RdDM). We studied their order of action within the RdDM pathway by testing their ability to target methylation in different mutants. We also evaluated ectopic siRNA biogenesis, RNA Polymerase V (Pol V) recruitment, targeted DNA methylation, and gene expression changes at thousands of ZF-RdDM targets. We found that co-targeting both arms of the RdDM pathway, siRNA biogenesis and Pol V recruitment, dramatically enhanced targeted methylation. This work defines how RdDM components establish DNA methylation, and enables new strategies for epigenetic gene regulation via targeted DNA methylation.
Overall design: 66 BS-PCR-seq samples, 39 small RNA-seq, 45 WGBS, 40 RNA-seq, 56 ChIP-seq
Less...| Accession | PRJNA513318; GEO: GSE124546 |
| Type | Umbrella project |
| Publications | Gallego-Bartolomé J et al., "Co-targeting RNA Polymerases IV and V Promotes Efficient De Novo DNA Methylation in Arabidopsis.", Cell, 2019 Feb 21;176(5):1068-1082.e19 |
| Submission | Registration date: 2-Jan-2019
University of California, Los Angeles |
| Relevance | Superseries |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 246 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 246 |
| GEO DataSets | 6 |
Co-targeting RNA Polymerases IV and V promotes efficient de novo DNA methylation in Arabidopsis encompasses the following 5 sub-projects:
| Project Type | Number of Projects |
| Epigenomics | 3 |
BioProject
accession | Organism | Title |
|---|
| PRJNA513321 | Arabidopsis thaliana | Co-targeting RNA Polymerases IV and V promotes efficient de novo DNA methylation in Arabidopsis [ChIP-seq] (University of California,...) | | PRJNA513322 | Arabidopsis thaliana | Co-targeting RNA Polymerases IV and V promotes efficient de novo DNA methylation in Arabidopsis [BS-PCR-seq] (University of California,...) | | PRJNA513323 | Arabidopsis thaliana | Co-targeting RNA Polymerases IV and V promotes efficient de novo DNA methylation in Arabidopsis [WGBS] (University of California,...) |
|
| Transcriptome or Gene expression | 2 |
BioProject
accession | Organism | Title |
|---|
| PRJNA513320 | Arabidopsis thaliana | Co-targeting RNA Polymerases IV and V promotes efficient de novo DNA methylation in Arabidopsis [small RNA-seq] (University of California,...) | | PRJNA513324 | Arabidopsis thaliana | Co-targeting RNA Polymerases IV and V promotes efficient de novo DNA methylation in Arabidopsis [RNA-seq] (University of California,...) |
|