BioProject

In our study, we generated and sequenced small RNA libraries from commercially available brain total RNA or human blood plasma samples. More...

In our study, we generated and sequenced small RNA libraries from commercially available brain total RNA or human blood plasma samples. These samples were generated with MAD-DASH, a method we developed employing CRISPR/Cas9 ribonucleoprotein targeting specific overabundant sequences such as adapter dimer or miRNAs to reduce these sequences from final libraries. We sequenced treated and untreated samples to demonstrate specificity, efficacy, and reproducibility of our MAD-DASH small-RNA sequencing protocol. Overall design: There are 42 fastq files in this submission, consisting of 12 fastqs from gel extracted small-RNA samples from human brain total RNA, 12 fastqs from bead cleanup (no gel extraction) small-RNA samples from human brain total RNA, and 18 fastqs from gel extracted small-RNA samples generated from human plasma RNA. Samples were performed in replicate (n=2 for brain RNA samples, n=3 for plasma RNA samples) with either no MAD-DASH CRISPR treatment, or MAD-DASH CRISPR treatment targeting either adapter-dimer, specific miRNAs, or a combination of adapter dimer and miRNAs. Also included is a count table after read processing. Less...