Nest fragments of three C. cumulans mounds were collected from Pinheiral grasslands, Rio de Janeiro, Brazil (GPS data: -22.
More...Nest fragments of three C. cumulans mounds were collected from Pinheiral grasslands, Rio de Janeiro, Brazil (GPS data: -22.5212761 and -43.9990613) and kept in polypropylene containers in a dark laboratory, at 23 ± 2°C and 60 ± 10% relative humidity. The experiments were performed using worker caste termites of undetermined age and with full guts.Total RNA was extracted using Trizol® reagent (Ambion™) following the manufacturer’s standard protocol, precipitated with 1.4 M NaCl and re-purified using the RNeasy Mini kit (Qiagen) according to the manufacturer's recommendations. RNA was subjected to RNase-free DNase I (Invitrogen™) treatment to remove any potential DNA contamination, and complementary DNA (cDNA) was synthesized with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™).
Less...| Accession | PRJEB25988 |
| Scope | Monoisolate |
| Submission | Registration date: 13-Jun-2018
UFRJ |
Project Data:
No public data is linked to this project. Any recently released data that cites this project will be linked to it within a few days.