High depth sequencing of 32 tumor formalin-fixed, paraffin-embedded (FFPE) samples from patients with HER2-positive breast cancer.
Samples were prepped and sequenced by Source Biosciences using an Agilent SureSelect panel using SureSelect QXT chemistry.
The custom library designed comprised of the ERBB-family genes and included 132 regions.
The total library size was 50 kbp, with average coverage of 98.87%.
The custom library included coding exons of the target genes.
The constructed libraries were sequenced using an Illumina MiSeq Sequencer.
For more information, please check the 'High Depth Sequencing' section under the methods in https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5342757/ Less...