BioProject

We aimed to compare the gene expression profiles of patients with degenerative MR in SR and AFib. More...

We aimed to compare the gene expression profiles of patients with degenerative MR in SR and AFib. We used Affymetrix human gene expression microarrays for each atrium sample. We chose most homogenous groups to compare, cause of the noise overshadows in high-throughput analysis when investigating complex diseases. Overall design: Left and right atrial tissue samples were obtained from patients with chronic primary severe MR in permanent AFib (n=15) and sinus rhythm (n=15). Transcriptomic analysis and bioinformatics have been done on all atrial tissues. Independent datasets from GEO were included in the analysis to confirm our findings. Real-time qPCR used to validate microarray results. Atrial tissues investigated via transmission electron microscopy for ultrasutructural changes. Less...