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Organizing biological data
overall design: Mouse embryonic fibroblasts from TNG_MKOS mice (Chantzoura et al., 2015. Stem Cell Reports. 2015; 5: 350–364) were overexpressed with ESRP1 and ESRP2. After 4 hours of infections with retroviral particles expressing ESRP1 and ESRP2, reprogramming was induced by adding doxycycline which induces the expression MKOS factors. For the first 5 days of reprogramming, mOrange positive cells and for day 15 Nanog-GFP positive cells were sorted and mRNA-seq samples were prepared as mentioned in Mittal et al., Nat Commun. 2017; 8:457. Less...
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