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Accession: PRJEB25216 ID: 475350

Combined bacterial and fungal intestinal microbiota analyses: Impact of storage conditions and DNA extraction protocols.

The human intestinal microbiota contains a vast community of microorganisms increasingly studied using high-throughput DNA sequencing. Standardized protocols for storage and DNA extraction from fecal samples have been established mostly for bacterial microbiota analysis. Here, we investigated the impact of storage and DNA extraction on bacterial and fungal community structures detected concomitantly.

Three feces from healthy adults were stored at -80°C as such or diluted in RNAlater® and subjected to 2 extraction protocols with mechanical lysis: the Powersoil® MoBio kit or the International Human Microbiota Standard Protocol Q. More...
AccessionPRJEB25216
ScopeMonoisolate
SubmissionRegistration date: 9-Jun-2018
INSTITUT PASTEUR
Project Data:
Resource NameNumber
of Links
Sequence data
SRA Experiments24
Other datasets
BioSample24
SRA Data Details
ParameterValue
Data volume, Mbases130
Data volume, Mbytes79

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