BioProject

Background: Schistosomiasis remains an important global public health problem that affects 285 million people in 78 countries. More...

Background: Schistosomiasis remains an important global public health problem that affects 285 million people in 78 countries. The immune response mechanisms of host–parasite interaction are complex, and miRNAs play a major role in modulation of development, homeostasis, as well as the function of immune cells. Results: In this study, an miRNA microarray was applied to investigate differences in miRNA expression in splenic B cells of normal and 13w post infected mice. In total, 43 miRNAs were detected in splenic B cells of the C57BL/6 mice before and after infection, including 33 miRNAs with up-regulated expression, and 10 miRNAs with down-regulated expression in mice 13w infection with schistosomes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the differentially expressed miRNAs revealed that many important biological pathways are triggered by schistosome infection in C57BL/6 mice, such as the Thyroid hormone signal pathway, Axon guidance, insulin signaling pathway, Pathways in cancer and Rap1 signaling pathway. The results reveal that miRNAs may be an important regulator of splenic B cells in the chronic phase of Schistosoma japonicum infection. Conclusions: The data presented here provide valuable information to increase understanding of the basic role of miRNAs in splenic B cells during the interaction of the S. japonicum with the host. This may be helpful in identifying the upstream molecular pathways controlling B cell specialization and provides a new platform of B cell manipulation to fine-tune their function. Overall design: In this study, an miRNA microarray was applied to investigate differences in miRNA expression in splenic B cells of uninfected and 13w post infected mice with schistosoma japonicum. There were 3 samples in the infected group and 3 samples in the uninfected group as control.Three independent experiments were performed using different mouse for each experiment. Less...