BioProject

The NKX3.1 homeobox gene functions in mitochondria to regulate oxidative stress To investigate the role of NKX3. More...

The NKX3.1 homeobox gene functions in mitochondria to regulate oxidative stress To investigate the role of NKX3.1 in regulation of oxidative stress, we employed transcriptome analysis of mouse prostate ( from 4 month old Nkx3.1+/+ and Nkx3.1-/- mice treated with paraquat), and human prostate cells ( RWPE1 cells engineered with empty vector (altered pTRIPZ), NKX3.1 wild type over-expression, and NKX3.1 (R52C) over-expression treated with paraquat). Mouse tissue or human cells were snap frozen for subsequent molecular analysis. Overall design: Analysis of transcriptome of human RWPE1 cells Human RWPE1 cells were engineered with empty vector (altered pTRIPZ) or NKX3.1 wild type over-expression or NKX3.1(R52C) overexpression, and treated with paraquat . Cells were snap frozen for subsequent molecular analysis Total RNA obtained from RWPE1 cells. Engineered RWPE1 cells were harvested and processed for RNA isolation and transcriptome analysis using the MagMAX RNA isolation kit (Ambion). For queries, please contact Cory Abate-Shen at cabateshen@columbia.edu Less...