T cell contacts with antigen-bearing dendritic cells (DCs) result in T cell activation. Whether this interaction has physiological consequences on DC function is largely unexplored. Results associated to this project indicate that antigen-dependent T cell contacts with DCs trigger anti-pathogenic programs in the latter, priming them against pathogen infection. Information is transmitted through extracellular vesicles (EVs) that contain genomic and mitochondrial DNA from the T cell to the DC. T cell transfer of EVs to DCs triggers transcellular antiviral responses that involve the cGAS/STING cytosolic DNA-sensing pathway and expression of IRF3-dependent interferon regulated genes. These events confer resistance to DCs to subsequent viral infections.
More...T cell contacts with antigen-bearing dendritic cells (DCs) result in T cell activation. Whether this interaction has physiological consequences on DC function is largely unexplored. Results associated to this project indicate that antigen-dependent T cell contacts with DCs trigger anti-pathogenic programs in the latter, priming them against pathogen infection. Information is transmitted through extracellular vesicles (EVs) that contain genomic and mitochondrial DNA from the T cell to the DC. T cell transfer of EVs to DCs triggers transcellular antiviral responses that involve the cGAS/STING cytosolic DNA-sensing pathway and expression of IRF3-dependent interferon regulated genes. These events confer resistance to DCs to subsequent viral infections. Together, our results reveal that T cells prime DCs through the transfer of exosomal DNA, supporting a specific role for antigen-dependent contacts in conferring protection to DCs against pathogen infection.
Less...| Accession | PRJNA472779 |
| Type | Umbrella project |
| Submission | Registration date: 23-May-2018
Bioinformatics Unit, CNIC |
| Relevance | Model Organism |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 10 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 10 |
| GEO DataSets | 1 |
This project encompasses the following 2 sub-projects:
| Project Type | Number of Projects |
Genome sequencingHighest level of assembly : SRA or Trace |
1
|
BioProject
accession | Assembly
level | Name | Title |
|---|
| PRJNA472354 | SRA or Trace | Analysis of mtDNA in primary T lymphoblast derived exosomes | Analysis of mtDNA in primary T lymphoblast derived exosomes (Centro Nacional de Investigaciones...) |
|
| Transcriptome or Gene expression | 1 |
BioProject
accession | Organism | Title |
|---|
| PRJNA432987 | Mus musculus | Analysis of changes in Dendritic cell (DC) gene expression upon exposition to T-cell Exosomes. (Bioinformatics Unit, CNIC) |
|