BioProject

A chromosome size-dependent bias in meiotic recombination is in place to ensure that homologous chromosome pairing occurs for all chromosomes, including the smallest ones. More...

A chromosome size-dependent bias in meiotic recombination is in place to ensure that homologous chromosome pairing occurs for all chromosomes, including the smallest ones. This bias is clearly detectable during the assembly of the meiotic protein axis, the structure that compacts meiotic chromosomes and promotes recombination. To investigate the origin of the size bias, we mapped genome-wide occupancy of the meiotic axis protein Red1 in yeast strains containing chromosome fusions. To allow optimal normalization, the experiments were performed in hybrid S288C/SK1 strains with fused S288C chromosomes normalized to native SK1 chromosomes in the same cells. Overall design: ChIP-seq analysis of Red1 DNA binding 3 hrs after induction of synchronous meiosis in SK1/S288C hybrid budding yeast strains containing fusions of SK1 chromosomes. Grant: March of Dimes FY16-208 Title: Meiotic segregation of small chromosomes PI: Andreas Hochwagen, New York University, New York, NY, United States Grant: NSF MCB-1616111 Title: Complete synthesis of designer eukaryotic genome, Sc2.0 PI: Jef D. Boeke, New York University Medical Center, New York, NY, United States Less...