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Genome Information for Schizosaccharomyces pombe
Deletion of pot1+ causes rapidly telomere degradation and chromosome circularization.
More...Deletion of pot1+ causes rapidly telomere degradation and chromosome circularization. We searched gene which becomes synthetically lethal with pot1 deletion. We obtained novel pik1 mutant that is synthetically lethal with pot1 disruption. We found PI4P in Golgi is reduced in pik1-1 mutant. To investigate the mechanism of the lethality of pot1 pik1-1 double mutant, we constructed nmt-pot1-aid pik1-1 strain, where using of Thiamine and Auxin in nmt-pot1-aid strain causes Pot1 shutoff that leads to immediate telomere loss and chromosome circularization, and found pik1-1 mutation does not affect telomere resection and chromosome circularization. Thus, our results suggest that pik1 is required for the maintenance of circular chromosomes.
Less...| Accession | PRJDB6467 |
| Data Type | Genome sequencing |
| Scope | Monoisolate |
| Organism | Schizosaccharomyces pombe 927[Taxonomy ID: 1264690] Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces; Schizosaccharomyces pombe; Schizosaccharomyces pombe 927 |
| Publications | - Published online: Sugihara A et al., "Mutation in fission yeast phosphatidylinositol 4-kinase Pik1 is synthetically lethal with defect in telomere protection protein Pot1", Biochemical and Biophysical Research Communications, 2018;496(4):1284-1290
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| Grants | - "PRESTO" (Grant ID N/A, Japan Science and Technology)
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| Submission | Registration date: 16-May-2018
Laboratory of Genome Regeneration, Center for Epigenetic Disease, The University of Tokyo, Institute of Molecular and Cellular Biosciences |
| Relevance | Model Organism |
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