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Genome Information for Mus musculus
Purpose: The goals of this study are to compare Next-generation sequencing (NGS)-derived transcriptome profiling (RNA-seq) in the lung of three tyeps of mice during influenza infection.
Methods: Total RNA from lung was extracted using a modified TRIzol protocol and spectrophometrically quantitated. Library preparation and sequencing were conducted using 3’ inTAG next-generation sequencing . Differential gene expression for day 6 post influenza infection was determined relative to mock inoculated mice.
Results: Differentially expressed genes were defined using p-value <0.01 and FDR-corrected p-value <0.1 cutoffs. We identified the transcripts in the lung of RIG-I-/-, MAVS -/- mice during influenza infection
Our study represents the first detailed analysis of lung transcriptomes of Wild Type , RIG-I-/-, MAVS -/- mice during influenza infection , with biologic replicates, generated by RNA-seq technology.
Overall design: Lung tissue mRNA profiles of influenza infected wild type (WT), RIG-I-/-, MAVS -/- mice at day 6 were generated by deep sequencing, n=6.
| Accession | PRJNA470625; GEO: GSE114232 |
| Data Type | Transcriptome or Gene expression |
| Scope | Multiisolate |
| Organism | Mus musculus[Taxonomy ID: 10090] Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus; Mus musculus |
| Publications | Wu W et al., "RIG-I Signaling via MAVS Is Dispensable for Survival in Lethal Influenza Infection In Vivo.", Mediators Inflamm, 2018;2018:6808934 |
| Submission | Registration date: 9-May-2018
Biostatistics, Virginia Commonwealth University |
| Relevance | Model Organism |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 36 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 36 |
| GEO DataSets | 1 |