EBF1 interacts with CNOT3 and histidine 240 of EBF1 is a critical residue for this interaction. We studied H240 dependent EBF1 occupancy and gene expression regulation by genome-wide ChIP and microarray analysis. We also performed RNASeq analysis in pro-B cells to study the role of Cnot3 in early B cells development. This SuperSeries is composed of the SubSeries listed below.
Overall design: Refer to individual Series
| Accession | PRJNA345460; GEO: GSE87637 |
| Type | Umbrella project |
| Publications | Yang CY et al., "Interaction of CCR4-NOT with EBF1 regulates gene-specific transcription and mRNA stability in B lymphopoiesis.", Genes Dev, 2016 Oct 15;30(20):2310-2324 |
| Submission | Registration date: 5-Oct-2016
Laboratory Rudolf Grosschedl, Cellular & Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics |
| Relevance | Superseries |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 8 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 8 |
| GEO DataSets | 4 |
GEO Data Details| Parameter | Value |
|---|
| Data volume, Spots | 330136 |
| Data volume, Processed Mbytes | 8 |
| Data volume, Supplementary Mbytes | 135 |
| Parameter | Value |
|---|
| Data volume, Gbases | 37 |
| Data volume, Mbytes | 14786 |
Interaction of CNOT3 with EBF1 regulates gene-specific transcription and mRNA stability in B lymphopoiesis encompasses the following 3 sub-projects:
| Project Type | Number of Projects |
| Epigenomics | 1 |
BioProject
accession | Organism | Title |
|---|
| PRJNA345468 | Mus musculus | Genome-wide occupancy of EBF1-wt & EBF1-H240A (Laboratory Rudolf Grosschedl,...) |
|
| Transcriptome or Gene expression | 2 |
BioProject
accession | Organism | Title |
|---|
| PRJNA345465 | Mus musculus | Gene expression profile of Cnot3(+/+) & Cnot3 (∆/∆) pro-B cells (Laboratory Rudolf Grosschedl,...) | | PRJNA345466 | Mus musculus | Gene expression profile of EBF1-wt & EBF1-H240A (Laboratory Rudolf Grosschedl,...) |
|