BioProject

The cellular and molecular mechanisms underlying neurodevelopmental conditions such as autism spectrum disorders have been studied intensively for decades. More...

The cellular and molecular mechanisms underlying neurodevelopmental conditions such as autism spectrum disorders have been studied intensively for decades. The ability to generate patient-specific induced pluripotent stem cells (iPSCs) now offers a novel strategy for modeling human disease. Recent studies have reported the derivation of iPSCs from patients with neurological disorders. The key challenge remains the demonstration of disease-related phenotypes and the ability to model the disease. Here we report a case study with signs of neurodevelopmental disorders (NDDs) harbouring chromosomal rearrangements that were sequenced using long-insert DNA paired-end tag (DNA-PET) sequencing approach. We identified the disruption of a specific gene, GTDC1. By deriving iPSCs from this patient and differentiating them into neural progenitor cells (NPCs) and neurons we dissected the disease process at the cellular level and observed defects in both NPCs and neuronal cells. We also showed that disruption of GTDC1 expression in wild type human NPCs and neurons showed similar phenotype as patient’s iPSCs. Finally, we utilized a zebrafish model to demonstrate a role for GTDC in development of the central nervous system. Our findings highlight the importance of combining sequencing technolgies with the iPSC technology for NDDs modelling that could be applied for personalized medicine. Overall design: Total RNA obtained from neurons derived from CD6 patient's iPSCs and shGTDC1 hESCs were compared to Control hiPSCs and shControl hESCs respectively. Less...