Long noncoding RNAs (lncRNAs) mediate important epigenetic regulation in a wide range of biological processes and diseases. We applied comprehensive analyses of RNA-seq and CAGE-seq (cap analysis of gene expression and sequencing) to characterize the dynamic changes in lncRNA expression in rhesus macaque (Macaca mulatta) brain in four representative age groups. We identified 18 anatomically diverse lncRNA modules and 14 mRNA modules representing spatial, age and sex specificities. Spatiotemporal- and sex-biased changes in lncRNA expression were generally higher than those observed in mRNA expression. A negative correlation between lncRNA and mRNA expression in cerebral cortex was observed and functionally validated. Our findings offer a fresh insight into spatial-, age- and sex-biased changes in lncRNA expression in macaque brain, and suggest that the changes represent a previously unappreciated regulatory system that potentially contributes to brain development and ageing.
More...Long noncoding RNAs (lncRNAs) mediate important epigenetic regulation in a wide range of biological processes and diseases. We applied comprehensive analyses of RNA-seq and CAGE-seq (cap analysis of gene expression and sequencing) to characterize the dynamic changes in lncRNA expression in rhesus macaque (Macaca mulatta) brain in four representative age groups. We identified 18 anatomically diverse lncRNA modules and 14 mRNA modules representing spatial, age and sex specificities. Spatiotemporal- and sex-biased changes in lncRNA expression were generally higher than those observed in mRNA expression. A negative correlation between lncRNA and mRNA expression in cerebral cortex was observed and functionally validated. Our findings offer a fresh insight into spatial-, age- and sex-biased changes in lncRNA expression in macaque brain, and suggest that the changes represent a previously unappreciated regulatory system that potentially contributes to brain development and ageing.
Overall design: Examination of 8 different brain regions in Rhsesu Macaque across the four ages (1-, 4-, 10- and 20-year old )
Less...| Accession | PRJNA343790; GEO: GSE87182 |
| Type | Umbrella project |
| Publications | - Xu K et al., "CircGRIA1 shows an age-related increase in male macaque brain and regulates synaptic plasticity and synaptogenesis.", Nat Commun, 2020 Jul 17;11(1):3594
- Liu S et al., "Annotation and cluster analysis of spatiotemporal- and sex-related lncRNA expression in rhesus macaque brain.", Genome Res, 2017 Sep;27(9):1608-1620
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| Submission | Registration date: 21-Sep-2016
Center for Genome Analysis, ABLife, Inc. |
| Relevance | Superseries |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 128 |
| Publications |
| PubMed | 2 |
| PMC | 2 |
| Other datasets |
| BioSample | 128 |
| GEO DataSets | 3 |
Annotation and cluster analysis of spatiotemporal- and sex-related lncRNA expression in Rhesus macaque brain encompasses the following 2 sub-projects:
| Project Type | Number of Projects |
| Transcriptome or Gene expression | 2 |
BioProject
accession | Organism | Title |
|---|
| PRJNA343788 | Macaca mulatta | Spatiotemporal- and sex-specific Dynamics of lncRNA expression links to brain development and aging in Rhesus Macaque (CAGE-seq) (Center for Genome Analysis,...) | | PRJNA338300 | Macaca mulatta | Spatiotemporal- and sex-specific Dynamics of lncRNA expression links to brain development and aging in Rhesus Macaque (RNA-seq) (Center for Genome Analysis,...) |
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