We first generated a single whole-genome BAC library
with long inserts (∼140 Kbp). This procedure is a
modification of the original haplotyping method pre-
viously described by Kitzman et al. (2011), that gen-
erates fosmid libraries with ∼40 Kbp inserts. Here
we used BAC clones. We then randomly
partitioned the library into pools such that each pool is
essentially a haploid mixture of clones derived from ei-
ther the maternal or paternal DNA at each genomic lo-
cation. High-throughput sequencing of each pool pro-
vides haplotype information for each clone in that
pool. Less...