BioProject

Diabetes is a complex metabolic syndrome characterized by prolonged high blood glucose levels. More...

Diabetes is a complex metabolic syndrome characterized by prolonged high blood glucose levels. It is known that diabetes is associated with an elevated risk of cancer, however, the underlying molecular mechanisms are largely unknown. In particular, it remains unclear as to how hyperglycemia may affect epigenetic checkpoints and tumor suppressor pathways, thus enabling oncogenic transformation. Here we show that long-term hyperglycemic conditions adversely impact the anti-tumor epigenetic mark DNA 5-hydroxymethylcytosine (5hmC) through direct regulation of the tumor suppressor and DNA 5mC hydroxymethylase, TET2. We identify TET2 as a novel substrate of the AMP-activated kinase (AMPK). The microarray data is used to study genes that are differentially regulated by glucose through TET2. Overall design: A2058-TET2WT, A2058-TET2M, and Mock cells were grown under high-g (4.5g/L) and normal-g (1g/L) DMEM medium for 7 days, with splitting every 2-3 days during growth. RNA was extracted and hybridized on Affymetrix microarrays. Less...