RNA polymerase II (Pol II) pauses downstream of the transcription initiation site before beginning productive elongation. This pause is a key component of metazoan gene expression regulation. Some promoters have a strong disposition for Pol II pausing and often mediate faster, more synchronous changes in expression. This requires multiple rounds of transcription and thus cannot rely solely on pause release. But it is unclear how pausing affects the initiation of new transcripts during consecutive rounds of transcription. Using our recently developed ChIP-nexus method, we find that Pol II pausing inhibits new initiation. We propose that paused Pol II helps prevent new initiation between transcription bursts, which may reduce noise.
Overall design: Refer to individual Series
| Accession | PRJNA339229; GEO: GSE85741 |
| Type | Umbrella project |
| Publications | Shao W et al., "Paused RNA polymerase II inhibits new transcriptional initiation.", Nat Genet, 2017 Jul;49(7):1045-1051 |
| Submission | Registration date: 17-Aug-2016
Zeitlinger lab, Stowers Institute for Medical Research |
| Relevance | Superseries |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 58 |
| Publications |
| PubMed | 1 |
| Other datasets |
| BioSample | 58 |
| GEO DataSets | 4 |
Paused RNA polymerase II inhibits new transcriptional initiation encompasses the following 3 sub-projects:
| Project Type | Number of Projects |
| Epigenomics | 3 |
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