BioProject

Alternative splicing (AS) is the main process that amplifies DNA information and is a crucial target of signaling cascades resulting from UV irradiation of human cells. More...

Alternative splicing (AS) is the main process that amplifies DNA information and is a crucial target of signaling cascades resulting from UV irradiation of human cells. The specific impact of UV-induced cyclobutane pyrimidine dimers (CPDs) at the transcriptional and AS levels has not been addressed. By using a CPD photolyase, a flavoenzyme that directly reverts CPDs, we analyze the contribution of this lesion to the expression program in human keratinocytes. Overall design: mRNA profiles of human keratinocytes (HaCaT cell line) expressing the CPD photolyase from Potorous tridactylus in three different conditions: 1. Control: Non treated cells and inactive photolyase. 2. UV treated cells: 15 J/m2 (UVC, 254 nm) and inactive photolyase, harvested 6 hours after UV irradiation and 3. Same as before but with an active photolyase due to exposure to white light for 2 hours. Less...