BioProject

To study how changes in the nuclear mechanical properties may modify the development of Ventilator-induced lung injury, mice of both genotypes were studied at baseline or after 2. More...

To study how changes in the nuclear mechanical properties may modify the development of Ventilator-induced lung injury, mice of both genotypes were studied at baseline or after 2.5 hours of mechanical ventilation (PIP 15cmH2O, ZEEP, 100 breaths/min, inspiratory:expiratory ratio 1:1, inpired oxygen fraction 0.21).After that, mice were sacrificed, the lungs estracted and gene expression measured in order to identify the differential gene expression depending on the different nuclear stifness. Overall design: 12 samples were analyzed. 6 from Zmpste24-deficient mice where 3 were baseline and 3 were submitted to mechanical ventilation and 6 wiltype where 3 were baseline and 3 were submitted to mechanical ventilation. Less...