In 1988, Richard Lenski started the LTEE, or long-term evolution experiment, in order to observe and analyze the process of evolution in action. Twelve populations were started from the same ancestral strain of Escherichia coli B, and they have been propagated in a glucose-limited minimal medium at 37 C ever since. Every day, 1% of each population is transferred into fresh medium, and this process has continued ever since. Every 500 generations, samples are frozen at -80 C for later analyses. This experiment has provided insights into the process of adaptation by natural selection, the repeatability of evolution, the dynamics of genome evolution, the origin of new functions, the evolution of mutation rates, and more.
| Accession | PRJNA294072 |
| Type | Umbrella project |
| Publications | Tenaillon O et al., "Tempo and mode of genome evolution in a 50,000-generation experiment.", Nature, 2016 Aug 11;536(7615):165-70 |
| Submission | Registration date: 27-Aug-2015
The University of Texas at Austin |
| Relevance | Evolution |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 2896 |
| Publications |
| PubMed | 7 |
| PMC | 6 |
| Other datasets |
| BioSample | 1964 |
This project encompasses the following 15 sub-projects:
| Project Type | Number of Projects |
| Genome sequencing |
11
4
15
|
BioProject
accession | Assembly
level | Name | Title |
|---|
| PRJNA896785 | SRA or Trace | E. coli transcriptome evolution on the path to a major metabolic innovation | E. coli transcriptome evolution on the path to a major metabolic innovation (The University of Texas at...) | | PRJNA188723 | SRA or Trace | Escherichia coli | Genomic analysis of a key innovation in an experimental Escherichia coli population. (The University of Texas at...) | | PRJNA295606 | SRA or Trace | Escherichia coli B str. REL606 | E. coli genome evolution over 50,000 generations (The University of Texas at...) | | PRJNA414462 | SRA or Trace | Escherichia coli Long-Term Evolution Experiment, Ara-3 Isolate Sequencing | Escherichia coli Long-Term Evolution Experiment, Ara-3 Isolate Sequencing (The University of Texas at...) | | PRJNA288762 | SRA or Trace | Escherichia coli REL606 | Mutation rate inferred from synonymous substitutions in a long-term evolution experiment with Escherichia coli (The University of Texas at...) |
List all 15 'Genome sequencing' projects...BioProject
accession | Assembly
level | Name | Title |
|---|
| PRJNA896785 | SRA or Trace | E. coli transcriptome evolution on the path to a major metabolic innovation | E. coli transcriptome evolution on the path to a major metabolic innovation (The University of Texas at...) | | PRJNA188723 | SRA or Trace | Escherichia coli | Genomic analysis of a key innovation in an experimental Escherichia coli population. (The University of Texas at...) | | PRJNA295606 | SRA or Trace | Escherichia coli B str. REL606 | E. coli genome evolution over 50,000 generations (The University of Texas at...) | | PRJNA414462 | SRA or Trace | Escherichia coli Long-Term Evolution Experiment, Ara-3 Isolate Sequencing | Escherichia coli Long-Term Evolution Experiment, Ara-3 Isolate Sequencing (The University of Texas at...) | | PRJNA288762 | SRA or Trace | Escherichia coli REL606 | Mutation rate inferred from synonymous substitutions in a long-term evolution experiment with Escherichia coli (The University of Texas at...) | | PRJNA257406 | SRA or Trace | Escherichia coli REL606 | Large Chromosomal Rearrangements during a Long-Term Evolution Experiment with Escherichia coli (The University of Texas at...) | | PRJNA262100 | SRA or Trace | Escherichia coli REL606 | Escherichia coli Long-term Evolution Experiment 500 and 1000 Generation Population Sequencing (The University of Texas at...) | | PRJNA270637 | SRA or Trace | Escherichia coli REL606 | Escherichia coli Long-Term Evolution Experiment, Ara-3 Whole-Population Sequencing (The University of Texas at...) | | PRJNA288811 | SRA or Trace | Escherichia coli REL606 | Synonymous genetic variation in natural isolates of Escherichia coli does not predict where synonymous substitutions occur in a long-term experiment (The University of Texas at...) | | PRJNA294164 | No data links | Escherichia coli REL606 | Genome evolution and adaptation in a long-term experiment with Escherichia coli. (The University of Texas at...) | | PRJNA294165 | No data links | Escherichia coli REL606 | Genome-wide mutational diversity in an evolving population of Escherichia coli (The University of Texas at...) | | PRJNA294166 | No data links | Escherichia coli REL606 | Escherichia coli REL606 Genome sequencing (The University of Texas at...) | | PRJNA295762 | No data links | Escherichia coli REL606 | Mutation accumulation evolution experiment (The University of Texas at...) | | PRJNA380528 | SRA or Trace | Escherichia coli REL606 | LTEE metagenomic sequencing over 60,000 generations (Harvard University) | | PRJNA649277 | SRA or Trace | Genomic evolution of antibiotic resistance is contingent on genetic background following a long-term experiment with Escherichia coli | Genomic evolution of antibiotic resistance is contingent on genetic background following a long-term experiment with Escherichia coli (Michigan State University) |
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