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Genome Information for Nicotiana tabacum
In order to learn the role of large numbers of genes (~20,000) a plant readily amenable to high-throughput transformation, plant propagation, and phenotypic screening was needed.
More...In order to learn the role of large numbers of genes (~20,000) a plant readily amenable to high-throughput transformation, plant propagation, and phenotypic screening was needed.
Nicotiana tabacum, a member of the Solanaceae, was chosen for four reasons: 1) the species is readily transformed by
Agrobacterium, 2) regeneration from transformed leaf discs produces uniform populations of explants, 3) the explants can be immediately transferred to soil and screened for phenotypic changes in the T
0 generation, and 4) the species exhibits post-transcriptional gene silencing in stably transformed transgenic plants. A second aim of the project was to test whether information gained by high-throughput screening could be transferred to
Arabidopsis from a dicot plant for which complete genome information is lacking.
Less...| Accession | PRJNA29351 |
| Data Type | Transcriptome or Gene expression |
| Scope | Monoisolate |
| Organism | Nicotiana tabacum[Taxonomy ID: 4097] Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana; Nicotiana tabacum |
| Publications (total 3) Less... | - Lein W et al., "Large-scale phenotyping of transgenic tobacco plants (Nicotiana tabacum) to identify essential leaf functions.", Plant Biotechnol J, 2008 Apr;6(3):246-63
More...- Lein W et al., "Large-scale phenotyping of transgenic tobacco plants (Nicotiana tabacum) to identify essential leaf functions.", Plant Biotechnol J, 2008 Apr;6(3):246-63
- Rotter A et al., "Adaptation of the MapMan ontology to biotic stress responses: application in solanaceous species.", Plant Methods, 2007 Sep 4;3:10
- Urbanczyk-Wochniak E et al., "Conversion of MapMan to allow the analysis of transcript data from Solanaceous species: effects of genetic and environmental alterations in energy metabolism in the leaf.", Plant Mol Biol, 2006 Mar;60(5):773-92
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| Submission | Registration date: 8-Dec-2004
Max-Planck-Gesellschaft |
Project Data:
| Resource Name | Number
of Links |
|---|
| Publications |
| PubMed | 3 |
| PMC | 1 |
No public data is linked to this project. Any recently released data that cites this project will be linked to it within a few days.