BioProject

Epithelial structure and function are governed by evolutionarily conserved signaling programs. More...

Epithelial structure and function are governed by evolutionarily conserved signaling programs. The Drosophila Activating transcription factor 3 (Atf3) prevents epithelial cell replacement during abdominal morphogenesis, yet its transcriptional targets are unknown. Here we demonstrate that Atf3 positively and negatively regulates target genes central to cytoskeleton dynamics and adhesion. Epithelial clones overexpressing Atf3 are enriched for basolateral proteins at the expense of apical identity. Strikingly, Atf3 functions downstream of the Scrib polarity module. Depleting either scrib or dlg1 induces atf3 expression, while loss of atf3 suppresses differentiation and polarity defects in cells deficient for the Scrib complex Overall design: Identification of Atf3 transcirptional targets in adult Drosospohila. In three Atf3 biological replicates, an Atf3::GFP fusion protein expressed from a germline-integrated, recombineered bacterial artificial chromosome (BAC) in an atf3 mutant backround. Three biological replicates which did not contain the BAC served as control. One chromatin input sample each from Atf3 and control samples served as a reference. Less...