The gastrointestinal tract contains a vast community of microbes that to this day remain largely unculturable, making studies in this area challenging.
More...The gastrointestinal tract contains a vast community of microbes that to this day remain largely unculturable, making studies in this area challenging. With the newly affordable advanced sequencing technology, important breakthroughs in this exciting field are now possible. However, standardized methods of sample collection, handling, and DNA extraction have yet to be determined. To help address this, we investigated the use of 5 common DNA extraction methods on fecal samples from 5 different species. Our data show that the method of DNA extraction impacts DNA concentration and purity, successful NGS amplification, and influences microbial communities seen in NGS output dependent on the species of fecal sample and the DNA extraction method used. These data highlight the importance of careful consideration of DNA extraction method used when designing and interpreting data from cross species studies.
Less...| Accession | PRJNA286763 |
| Data Type | Raw sequence reads |
| Scope | Environment |
| Organism | gut metagenome[Taxonomy ID: 749906] unclassified sequences; metagenomes; organismal metagenomes; gut metagenome |
| Grants | - "Postdoctoral Training in Comparative Medicine" (Grant ID T32 OD011126, National Institutes of Health)
- "Mutant Mouse Resource and Research Center" (Grant ID U42 OD010918, National Institutes of Health)
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| Submission | Registration date: 11-Jun-2015
University of Missouri Metagenomics Center |
| Related Resources | |
| Relevance | Gastrointestinal microbiota |
Project Data:
| Resource Name | Number
of Links |
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| BioSample | 1 |
No public data is linked to this project. Any recently released data that cites this project will be linked to it within a few days.