See
Genome Information for Mycolicibacterium smegmatis
The experiment was carried out to study the effect of loss of (p)ppGpp or cyclic di-GMP on the gene expression pattern of Mycobacterium smegmatis MC2 155 strains. The cells were cultured in MB7H9 broth containing 0.2% glycerol and 0.05% Tween 80. The transcriptome analysis was performed using GeneSpring GX12 software. Fold changes were calculated with respect to the median expression of wild type replicates. The number of differentially expressed genes (p <0.1) in rel knockout were 417 whereas those in dcpA knockout were 149. Earlier studies had shown that rel knockout and dcpA knockout had shown lower levels of glycopeptidolipids and polar lipids in the cell wall. The microarray data also corraborates our current findings.
Overall design: Organism : Mycobacterium smegmatis, Agilent Custom Mycobacterium smegmatis Gene Expression M.smegmatis_gxp_8X15K (AMADID: 029929) designed by Genotypic Technology Private Limited.
| Accession | PRJNA286176; GEO: GSE69681 |
| Data Type | Transcriptome or Gene expression |
| Scope | Multiisolate |
| Organism | Mycolicibacterium smegmatis MC2 155[Taxonomy ID: 246196] Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycolicibacterium; Mycolicibacterium smegmatis; Mycolicibacterium smegmatis MC2 155 |
| Publications | Gupta KR et al., "Regulation of Growth, Cell Shape, Cell Division, and Gene Expression by Second Messengers (p)ppGpp and Cyclic Di-GMP in Mycobacterium smegmatis.", J Bacteriol, 2016 May;198(9):1414-22 |
| Submission | Registration date: 9-Jun-2015
108, Molecular Biphysics Unit, Indian Institute of Science |
| Relevance | Unknown |
Project Data:
| Resource Name | Number
of Links |
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| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| GEO DataSets | 1 |
GEO Data Details| Parameter | Value |
|---|
| Data volume, Spots | 41388 |
| Data volume, Processed Mbytes | 2 |
| Data volume, Supplementary Mbytes | 18 |