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Accession: PRJNA284242 ID: 284242

Xenopus laevis (African clawed frog)

The identification of differentially expressed genes between animal and vegetal blastomeres in Xenopus laevis

See Genome Information for Xenopus laevis
To identify asymmetrically localized maternal mRNAs along the animal-vegetal axis in cleavage Xenopus embryos, we isolated animal and vegetal blastomeres at 8-cell stage, extracted the maternal mRNA respectively and analyzed them by RNA-seq technology. We identified 43 maternal transcripts significantly enriched in the vegetal region (FDR<0.05) by R/Bioconductor package DESeq Overall design: RNAseq of animal and vegetal blastomeres with 2 biological replicates
AccessionPRJNA284242; GEO: GSE68972
Data TypeTranscriptome or Gene expression
ScopeMultiisolate
OrganismXenopus laevis[Taxonomy ID: 8355]
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus; Xenopus laevis
PublicationsSun G et al., "Small C-terminal Domain Phosphatase 3 Dephosphorylates the Linker Sites of Receptor-regulated Smads (R-Smads) to Ensure Transforming Growth Factor β (TGFβ)-mediated Germ Layer Induction in Xenopus Embryos.", J Biol Chem, 2015 Jul 10;290(28):17239-49
SubmissionRegistration date: 18-May-2015
Michael Q. Zhang, Biology, Tsinghua Univeristy
RelevanceModel Organism
Project Data:
Resource NameNumber
of Links
Sequence data
SRA Experiments4
Publications
PubMed1
PMC1
Other datasets
BioSample4
GEO DataSets1
GEO Data Details
ParameterValue
Data volume, Supplementary Mbytes1
SRA Data Details
ParameterValue
Data volume, Gbases36
Data volume, Mbytes24118

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