BioProject

We have integrated dynamic RXRa binding, chromatin accessibility and promoter epigenetic status with the transcriptional activity inferred from RNA polymerase II mapping and transcription profiling. More...

We have integrated dynamic RXRa binding, chromatin accessibility and promoter epigenetic status with the transcriptional activity inferred from RNA polymerase II mapping and transcription profiling. This demonstrated a temporal organization structure, in which early events are preferentially enriched for common GRNs, while cell fate specification is reflected by the activation of late programs in a cell-type specific manner. Furthermore, significant differences in cell lines' promoter status of genes associated with cell-line specific programs were inferred. Finally, a variety of transcription factors (TFs) playing a direct role in the signal transduction cascade downstream of the RXR/RAR initiated wiring were identified, several of them commonly regulated in both model systems, but in addition cell-type specific TF drivers were also identified. Overall design: time series gene expression assessed over the retinoic acide induced differentiation in P19 and F9 embryonal carcinoma cells. 5 timepoints over four different treatment conditions (ATRA; RARa agonist BMS753; RARb agonist BMS641 and the RARg agonist BMS961) assessed in P19 cells and compared with those assessed in F9 cells described in a previous study: GSE30537 Less...