BioProject

Excisional biopsies of lymph nodes involved by follicular lymphoma (FL) were obtained from previously-untreated, advanced-stage patients to generate heterohybridomas and idiotype vaccine for a clinical trial (PMID: 21632504). More...

Excisional biopsies of lymph nodes involved by follicular lymphoma (FL) were obtained from previously-untreated, advanced-stage patients to generate heterohybridomas and idiotype vaccine for a clinical trial (PMID: 21632504). Residual, viably-cryopreserved suspensions of disaggregated cells were magnetically sorted by negative selection into nominal B (CD3-depleted) and non-B (NB; CD19- and CD20-depleted) fractions. Total RNA was extracted from each fraction for separate gene expression profiling (GEP). Technically-satisfactory GEP results were obtained from both fractions for 43 patients. Overall design: GEP data from the NB fraction were used to identify signatures of immune cell types and biological processes in the NB fraction, through the technique of correlation matrix analysis (CMA). B and NB fraction data were also cross-correlated, by CMA and other means, to implicate immune cell types, genes, and biological processes involved in interactions between tumor B cells and the immune microenvironment in FL. Negatively-enriched B or non-B cells, as indicated, from untreated follicular lymphoma. 7-digit number is the unique patient identifier. Suffix number, when present, indicates which one of the duplicate arrays that were used for the same patient. Less...