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Genome Information for Homo sapiens
Purpose: In this study, we show that DNA damage-activated AKT phosphorylates threonine 45 of core histone H3 (H3-T45)
Result: By genome-wide chromatin immunoprecipitation sequencing (ChIP-seq) analysis, H3-T45 phosphorylation was distributed throughout DNA damage-responsive gene loci, particularly immediately after the transcription termination site
Conclusion: AKT-mediated phosphorylation of H3-T45 regulates the processing of the 3′ end of DNA damage-activated genes to facilitate transcriptional termination
Overall design: MCF10A cells were ChIPed with anti-phosphorylated H3-T45, anti-phosphorylated RNA Pol II-S2 and S5, and anti-H3-K36me3.
| Accession | PRJNA280724; GEO: GSE67699 |
| Data Type | Epigenomics |
| Scope | Multiisolate |
| Organism | Homo sapiens[Taxonomy ID: 9606] Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo; Homo sapiens |
| Publications | Lee JH et al., "AKT phosphorylates H3-threonine 45 to facilitate termination of gene transcription in response to DNA damage.", Nucleic Acids Res, 2015 May 19;43(9):4505-16 |
| Submission | Registration date: 9-Apr-2015
National Creative Research Center for Epigenome Reprogramming Network, Biomedical Sciences, Seoul National University College of Medicine |
| Relevance | Medical |
Project Data:
| Resource Name | Number
of Links |
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| Sequence data |
| SRA Experiments | 5 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 5 |
| GEO DataSets | 1 |