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Genome Information for Drosophila melanogaster
JAK/STAT pathway plays important roles in controlling Drosophila intestinal homeostasis and regulating the ISC proliferation and differentiation. However,the downstream targets of its transcription factor-STAT92E remain largely unknown.To further identify the regualtory mechanisms of the JAK/STAT pathway in controlling intestinal homeostasis,we performed the ChIP-Seq assay with mouse raised STAT92E antibody using JAK/STAT signaling highly activated adult intestines.Through the ChIP assay, we have identified over 1000 significant peaks (p<0.01) around the putative targets.The well-characterized JAK/STAT downstream targets including Domeless,Socs36E,STAT92E and chinmo were identified in our ChIP assay,indicating that our experiment is workable to identify novel JAK/STAT downstream targets in adult intestines.This work will provide insights into our understanding of regulatory mechanisms of JAK/STAT signaling during Drosophila intestinal development.
Overall design: Identify the ChIP peaks of STAT92E antibody using JAK/STAT signaling highly actived Drosophila adult intestines, compared with input libaray as the control
| Accession | PRJNA279555; GEO: GSE67346 |
| Data Type | Epigenomics |
| Scope | Multiisolate |
| Organism | Drosophila melanogaster[Taxonomy ID: 7227] Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora; Drosophila melanogaster |
| Publications | Ren W et al., "Windpipe controls Drosophila intestinal homeostasis by regulating JAK/STAT pathway via promoting receptor endocytosis and lysosomal degradation.", PLoS Genet, 2015 Apr;11(4):e1005180 |
| Submission | Registration date: 27-Mar-2015
State Key Laboratory of Biomembrane and Membrane Biotechnology, INSTITUTE OF ZOOLOGY, CHINESE ACADEMY OF SCIENCES |
| Relevance | Model Organism |
Project Data:
| Resource Name | Number
of Links |
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| Sequence data |
| SRA Experiments | 2 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 2 |
| GEO DataSets | 1 |